ethyl 3-(4-(methylamino)-3-nitro-N-(pyridin-2-yl)benzamido)propanoate(CAS# 429659-01-8)

Mawu Oyamba

Ethyl 3-(4-(methylamino)-3-nitro-N-(pyridin-2-yl)benzoylamido)propionate, wotchedwanso MTT (Meta-Toluidine ortho-Toluenesulphonic acid triazine ester), ndi mankhwala. Lili ndi zotsatirazi:

Maonekedwe: Cholimba choyera

Kusungunuka: Kusungunuka mu mowa, ketoni ndi chloroform

Ntchito zazikulu za MTT ndi izi:

Kafukufuku wachilengedwe: MTT imagwiritsidwa ntchito kwambiri kudziwa kuchuluka kwa ma cell komanso kukula kwa maselo. Amasintha MTT kukhala chinthu cha buluu chomwe chimasungunuka m'maselo pochichepetsa, chomwe chingagwiritsidwe ntchito kudziwa momwe selo lingakhalire.

Kawirikawiri, njira yokonzekera MTT ikhoza kuchitidwa ndi izi:

Methylaniline imapangidwa ndi 2-nitrobenzoyl chloride kuti ipeze 3-(4-methylaminobenzoyl) benzoyl chloride.

Zomwe zimapezedwa mu gawo 1 zimachitidwa ndi 2-pyridone kupanga 3-(4-methylaminobenzoylamido) benzoyl chloride.

Chopangidwa chomwe chimapezedwa mu gawo 2 chimachitidwa ndi ethyl 3-aminopropionate kupanga ethyl 3-(4-methylaminobenzoylamido) propionate.

Chomaliza, MTT, chimayeretsedwa ndi crystallization kapena njira zina.

Chidziwitso cha Chitetezo: MTT ndi chinthu chapoizoni, ndipo tiyenera kusamala kuti chigwiritsidwe ntchito ndikuchitaya moyenera. Zida zodzitetezera zoyenera monga magolovesi, magalasi, ndi zokutira za labotale ziyenera kuvalidwa panthawi yogwira ntchito. Pewani kutulutsa mpweya, kukhudza khungu, ndi kudya. Mukamagwiritsa ntchito kapena kutaya zinyalala, tsatirani njira zoyendetsera chitetezo, ndipo tayani mwachangu ndi kuyeretsa zomwe zachitika mwadzidzidzi. Mukapuma kapena kukhudza khungu, pitani kuchipatala mwamsanga.