FMOC-Arg(Pbf)-OH (CAS# 154445-77-9)
Ngozi ndi Chitetezo
| Zizindikiro Zowopsa | Xi - Zosangalatsa |
| Zizindikiro Zowopsa | 36/37/38 - Zokwiyitsa maso, kupuma komanso khungu. |
| Kufotokozera Zachitetezo | S26 - Mukakhudza maso, yambani nthawi yomweyo ndi madzi ambiri ndikupempha uphungu wachipatala. S36 - Valani zovala zoyenera zodzitetezera. |
| WGK Germany | 1 |
| HS kodi | 2935 90 90 |
| Kalasi Yowopsa | ZOKWIYA |
Mawu Oyamba
Gulu loteteza FMOC ndi gulu loteteza amino acid lomwe limateteza gulu la amino la arginine. Zotsatirazi ndikuyambitsa zina mwazinthu, ntchito, njira zokonzekera, ndi chidziwitso chachitetezo cha Fmoc-Protective Radical:
Ubwino:
Gulu loteteza FMOC ndi gulu loteteza lomwe limateteza magulu amino amino. Itha kuchitapo kanthu ndi gulu la amino mu arginine kudzera mu esterification reaction kupanga Fmoc-arginine ester, kuti akwaniritse cholinga choteteza gulu la amino. Pali magulu onunkhira pa gulu la Fmoc-protecting gulu lomwe limayatsa kwambiri kuwala kwa UV, komwe kumalola kuti gulu loteteza Fmoc lichitidwe ndi kuwala kwa UV kapena njira zama mankhwala.
Gwiritsani ntchito:
Magulu oteteza FMOC amagwiritsidwa ntchito kwambiri popanga peptide komanso kaphatikizidwe kagawo kolimba. Ikhoza kuteteza gulu la arginine amino kuti liteteze zotsatira zake panthawi ya kaphatikizidwe. Mu kaphatikizidwe ka peptide, gulu loteteza Fmoc limatha kuchotsedwa ndi zinthu zamchere, kulola kuti kaphatikizidwe ka polypeptides kupitirire.
Njira:
Gulu loteteza Fmoc likhoza kukonzedwa ndi momwe Fmoc-Cl ndi arginine amachitira. Fmoc-Cl ndi reagent ya acidic kwambiri yomwe imagwirizana ndi gulu la amino mu arginine kupanga Fmoc-arginine ester. Zimene zimachitika kawirikawiri ikuchitika Mowa firiji kwa ayezi kusamba kutentha.
Zambiri Zachitetezo:
Ma radicals oteteza FMOC ndi otetezeka kuti agwiritsidwe ntchito m'ma labotale, koma izi ziyenera kudziwidwa:
- FMOC-CL ndi mankhwala osokoneza bongo komanso owopsa, chisamaliro chiyenera kutengedwa kuti musagwirizane ndi khungu, kupuma kapena kuyamwa. Gwirani ntchito pamalo olowera mpweya wabwino ndi kuvala zida zoyenera zodzitetezera.
- FMOC-Protective Base ili ndi mphamvu yoyamwa mwamphamvu kwa cheza cha ultraviolet, choncho chisamaliro chiyenera kutengedwa kuti tipewe kukhudzana ndi khungu ndi maso pakugwiritsa ntchito, ndikupewa kuwala kwamphamvu.
- Chitetezo champhamvu cha asidi hydrolysis monga pentafluorophenylcarboxylic acid (TFA) nthawi zambiri chimagwiritsidwa ntchito pochotsa magulu a Fmoc-zoteteza, ndipo m'pofunika kudziwa kuti nthunzi ya TFA ikhoza kuwononga, choncho m'pofunika kugwira ntchito bwino. malo olowera mpweya.
