fmoc-O-tert-butyl-D-tyrosine (CAS# 118488-18-9)
Fmoc-O-tert-butyl-D-tyrosine ndi amino acid yotetezedwa yomwe imagwiritsidwa ntchito kwambiri. Zotsatirazi ndizofotokozera za chikhalidwe chake, kagwiritsidwe ntchito, kapangidwe ndi chitetezo:
Chilengedwe:
Fmoc-O-tert-butyl-D-tyrosine ndi cholimba cha crystalline choyera. Ndi organic pawiri ndi mankhwala chilinganizo C30H31NO7 ndi molecular kulemera kwa 521.57g/mol. Pawiriyi ndi yochokera ku tyrosine momwe gulu la amino limanyamula gulu loteteza Fmoc (9-fluorofluorenylformyl) ndipo gulu la carboxylic acid limapangidwa ndi O-tert-butyl.
Gwiritsani ntchito:
Fmoc-O-tert-butyl-D-tyrosine amagwiritsidwa ntchito ngati amino acid otetezedwa mu peptide synthesis. Mwa kulumikiza gulu loteteza la Fmoc ku gulu la amino, zotsatira za mbali zosafunikira zitha kupewedwa panthawi ya kaphatikizidwe. Amagwiritsidwa ntchito kwambiri popanga gawo lolimba ndipo angagwiritsidwe ntchito popanga ma polypeptides ndi mapuloteni.
Njira Yokonzekera:
Kukonzekera kwa Fmoc-O-tert-butyl-D-tyrosine kawirikawiri kumachitika ndi kaphatikizidwe ka mankhwala. Choyamba, tyrosine imayendetsedwa ndi Fmoc-Cl (9-fluorofluorenylcarbonyl chloride) kuti ipange Fmoc-O-tyrosine. Cesium tert-butyl bromide imawonjezeredwa ku zomwe zimachititsa kuti esterify gulu la carboxylic acid kuti lipange Fmoc-O-tert-butyl-D-tyrosine. Pomaliza, choyeracho chimapezedwa ndi masitepe a crystallization, kutsuka ndi kuyanika.
Zambiri Zachitetezo:
Fmoc-O-tert-butyl-D-tyrosine ndi gulu lokhazikika pansi pazikhalidwe zodziwika bwino ndipo alibe kugwedezeka koonekera pa kutentha. Mukamagwiritsa ntchito, ndikofunikira kutsatira njira zotetezera ma labotale, kuvala zida zoyenera zodzitetezera, ndikupewa kukhudzana ndi khungu ndi maso. Pogwira kapena kusunga, iyenera kusungidwa pamalo owuma, ozizira komanso kutali ndi moto ndi oxidizing agents. Panthawi imodzimodziyo, kukhudzana ndi ma okosijeni amphamvu ndi ma asidi amphamvu kuyenera kupewedwa kuti mupewe zoopsa. Funsani thandizo lachipatala mwamsanga ngati mutamwa mowa kapena kukhudzana mwangozi ndi mankhwalawa.
