fmoc-L-4-hydroxyproline (CAS# 88050-17-3)

Mawu Oyamba

Fmoc-L-hydroxyproline (Fmoc-Hyp-OH) ndi yochokera ku amino acid yokhala ndi zotsatirazi ndikugwiritsa ntchito:

Ubwino:

- Maonekedwe: ufa wonyezimira woyera kapena wotuwa

- Kusungunuka: Kusungunuka mu zosungunulira organic monga DMF, DMSO ndi methanol

- pKa mtengo: 2.76

Gwiritsani ntchito:

- Fmoc-Hyp-OH imagwiritsidwa ntchito makamaka popanga peptide ndi kaphatikizidwe ka peptide mu kaphatikizidwe kolimba.

- Imakhala ngati gawo la gulu loteteza kuteteza magulu am'mbali ogwira ntchito amino acid panthawi yolimba-gawo kaphatikizidwe kuti apewe zosayembekezereka ndikusunga kusankha.

Njira:

Fmoc-Hyp-OH ikhoza kukonzedwa pochita ma Fmoc-amino acid ndi L-hydroxyproline muzosungunulira zoyenera. Zomwe zimachitikira nthawi zambiri zimaphatikizapo kutentha koyenera komanso chothandizira choyenera, monga N,N-dimethylpyrrolidone (DMAP). Chotsatiracho chimayeretsedwa ndi masitepe monga mvula, kuchapa, ndi kuyanika.

Zambiri Zachitetezo:

- FMOC-HYP-OH ndi organic pawiri ndipo ayenera kusamalidwa motsatira ma laboratory chitetezo protocols.

- Fumbi limatha kutulutsa mpweya ndikukhudzana ndi khungu, choncho samalani kuti musapusitsidwe kapena kukhudza.

- Panthawiyi, zida zoyenera zodzitetezera monga magolovesi a labotale, zoteteza maso, zodzitchinjiriza, ndi zina zotere ziyenera kuvala.

- Iyenera kusungidwa yosindikizidwa mwamphamvu pamalo owuma, ozizira, kutali ndi moto ndi zinthu zomwe zimatha kuyaka.