N(alpha)-fmoc-N(epsilon)-(2-chloro-Z)-L-lysine(CAS# 133970-31-7)

Fmoc-(2-chlorobenzyloxycarbonyl) lysine ndizochokera ku amino acid zomwe zimagwiritsidwa ntchito motere:1. Maonekedwe: ufa woyera wa crystalline;
2. chilinganizo cha maselo: C26H24ClNO5;
3. Kulemera kwa maselo: 459.92g / mol;
4. Kusungunuka: Kusungunuka mu zosungunulira za organic monga dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), dichloromethane, etc., zosasungunuka m'madzi;
5. Malo osungunuka: pafupifupi 170-175 ° C. Kugwiritsa ntchito kwakukulu kwa Fmoc-(2-chlorobenzyloxycarbonyl) lysine ndi gulu loteteza ndi loyambitsa mu kaphatikizidwe ka polypeptides. Gulu lake la carboxyl limatha kutsegulidwa kuti lipange ester, yomwe kenako imakumana ndi condensation ndi zotsalira za amino acid kuti apange unyolo wa polypeptide. Gulu la Fmoc likhoza kuchotsedwa mosavuta pambuyo pomaliza kuti awonetsere chitetezo cha amino moiety.

Njira yokonzekera Fmoc-(2-chlorobenzyloxycarbonyl) lysine nthawi zambiri imakhala ndi izi:

1. kuchita lysine ndi N-hydroxybutyrimide (Pbf) kuyambitsa gulu loteteza;
2. kuchitapo kanthu kochokera ku lysine-Pbf ndi 2-chlorobenzyl mowa kupanga Fmoc-(2-chlorobenzyloxycarbonyl) lysine;
3. Mankhwalawa amachotsedwa ndi zosungunulira zoyenera ndikuyeretsedwa ndi crystallization kuti apeze mankhwala oyera.

Pazambiri zachitetezo, Fmoc-(2-chlorobenzyloxycarbonyl) lysine ndi mankhwala ndipo njira zotetezera ziyenera kutengedwa. Zida zodzitetezera zoyenera, monga magolovesi a labu, magalasi, ndi zovala za labu, ziyenera kuvalidwa panthawi yoyesera. Pewani kutulutsa ufa kapena mankhwala, pewani kukhudzana ndi khungu ndi maso. Ngati mwakumana mwangozi, muzimutsuka nthawi yomweyo ndi madzi ambiri ndikupempha thandizo lachipatala. Onetsetsani kuti ikugwiritsidwa ntchito pamalo otetezeka a labotale ndikusungidwa bwino kuti mupewe ngozi.